Question: Student separating A and B by liquid chromatography. A 30 cm long C18, 1.7 micron particle sized, packed column was used. The retention factors A=

Student separating A and B by liquid chromatography. A 30 cm long C18, 1.7 micron particle sized, packed column was used. The retention factors A= 13.25 and B= 16.98. Elution of the solvent was 0.05 mins. Peak width A = 0.25 and B= 0.36 minutes. -calculate capacity factor k for A and B - calculate relative retention for A and B. Calculate resolution for A and B - what would happen to the resolution is the column was changed to one of that employed 3-micron particles instead. The others parameters remain the same. Explain/equation to help

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