summarize the procedure for the experiment in bullet points

Next, spot a plate with the standards which you have chosen, your unknown, and a mixture of the standards prepared by spotting them one over the other. Develop the plate and draw a replica of it in your Results section. When you are sure of the identities of the compounds in your unknown mixture, calculate the R, value for all spots on the last plate. Record these values as well as a replica of the TLC plate. NOTE: /f you have problems, you may require more plates than those described above. If so, record information only for those plates which run properly and con- tribute to your determination of the identity of the components of your unknown mixture. Also, some compound's may streak when run on TLC plates. If they do so consistently, this is useful descriptive information about the spots that can be used to help identify the compounds. TLC Analysis of Analgesic Drugs Analgesics, compounds that relieve pain, range from aspirin to morphine and related narcotics. In addition to aspirin, several other chemically similar compounds are widely used in nonprescription analgesic tablets. Among these are acetaminophen, ibuprofen, and salicylamide. Caffeine is sometimes added to these formulations to overcome drowsiness. Other ingredients may be added for different therapeutic effects, such as an antispasmodic or compounds that have a slight sedative action. In addition to the active ingredients, the tablets contain other substances that act as binders or enhance dissolution. In this experiment, you will obtain as an unknown an over-the-counter analgesic drug. You are to identify the unknown drug by TLC comparison with these known reference compounds: aspirin (acetylsalicylic acid), acetaminophen (4'-acetamido- phenol or 4'-hydroxyacetanilide), and caffeine. Your unknown analgesic will be one of those listed below; the chemical structures of the ingredients follow. Ingredients Anacin Aspirin and caffeine Bayer aspirin Aspirin Excedrin Acetaminophen, aspirin, and caffeine Tylenol Acetaminophen oO I 4 an' cts 0 CH, Oy OH | ae N o ah Ai hei CH3 O " OH CH; Aspirin Acetaminophen Caffeine 67