Tabulate the transformation efficiencies as transformants per 108 cells (group and class) determined under the varying conditions. Include one sample calculation and explain all results obtained. What are the consequences of omitting Carrier DNA, Heat Shock and PEG on the number of transformants produced? Table 1. Shows the transformation Treatments Control Normal No Carrier No PEG No HS F & M Average 0. 00 1 130 .00 0.00 Class Average 0. 00 7 70.14 1.73 0.13 11.535 Cell Density of yeast = 3.46x107 cells/ml Used: 1.156ml/tube (3.46x107 cells/ml) (1.156ml) = 4.0x10' cells (4.0x107 cells)/(0.5ml) = (8.0x107 cells/ml) (0.1ml plated) (# transformants) / ((8.0x10 cells) == (-X transformants) / (10 cells) "X' transformants = [(# transformants) / (8.0x10 cells)] (10%cells) Normal (transformants) = [(113 transformants) / (8.0x10' cells)] (10%cells) Normal (transformants) = 1412.5 transformants/ 10 cells. Table 2 Treatments F & M Average (transformants/ 10 cells) Class Average Control 0.00 Normal I 1412.5 No Carrier | No PEG | No HS 0.00 0.00 0.00 9626.75 21.625 1.625 144.125 Tabulate the transformation efficiencies as transformants per 108 cells (group and class) determined under the varying conditions. Include one sample calculation and explain all results obtained. What are the consequences of omitting Carrier DNA, Heat Shock and PEG on the number of transformants produced? Table 1. Shows the transformation Treatments Control Normal No Carrier No PEG No HS F & M Average 0. 00 1 130 .00 0.00 Class Average 0. 00 7 70.14 1.73 0.13 11.535 Cell Density of yeast = 3.46x107 cells/ml Used: 1.156ml/tube (3.46x107 cells/ml) (1.156ml) = 4.0x10' cells (4.0x107 cells)/(0.5ml) = (8.0x107 cells/ml) (0.1ml plated) (# transformants) / ((8.0x10 cells) == (-X transformants) / (10 cells) "X' transformants = [(# transformants) / (8.0x10 cells)] (10%cells) Normal (transformants) = [(113 transformants) / (8.0x10' cells)] (10%cells) Normal (transformants) = 1412.5 transformants/ 10 cells. Table 2 Treatments F & M Average (transformants/ 10 cells) Class Average Control 0.00 Normal I 1412.5 No Carrier | No PEG | No HS 0.00 0.00 0.00 9626.75 21.625 1.625 144.125