Question: The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples The following results was obtained: Table
The Bradford method described in the Background section was used to determine protein concentrations of known and unknown samples
The following results was obtained:
Table 1: Absorbance at 595nm obtained for different concentrations of protein standards using the Bradford method
| Protein (mg/ml) | 0.00 | 0.125 | 0.25 | 0.50 | 0.75 | 1.00 | 1.50 | 2.00 | Unknown |
|
Abs. BSA |
0 |
0.130 |
0.350 |
0.620 |
0.870 |
1.120 |
1.400 |
1.600 | 0.699 |
|
Abs. BIg |
0 |
0.080 |
0.230 |
0.460 |
0.520 |
0.410 |
0.510 |
0.700 |

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