The BSA standard solution with the concentration of 0.5 mg/mL was used to produce standard curve for
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The BSA standard solution with the concentration of 0.5 mg/mL was used to produce standard curve for protein assay based on Bradford method The test tubes contains BSA solutions was prepared: 0, 20, 40, 60, 80, 100 ul. The reading of the absorbance for each test tube after adding the Bradford reagent was 0, 0.05, 0.09. 0.14, 0.19 and 0.22. If 20 ul of protein solution with unknown concentration were taken and mixed with 80 ul. water. Then, 10 L of this mixture was reacted with Bradford reagent and the absorbance reading was 0.08. What is unknown protein solution concentration before dilution?
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Managing Supply Chain and Operations An Integrative Approach
ISBN: 978-0132832403
1st edition
Authors: Thomas Foster, Scott E. Sampson, Cynthia Wallin, Scott W Webb
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