Question: The diffusion coefficients for two proteins differ by a factor of 2, but the two proteins exhibit the exact same capillary electrophoresis retention (or migration)
The diffusion coefficients for two proteins differ by a factor of 2, but the two proteins exhibit the exact same capillary electrophoresis retention (or migration) times. How do they differ? Can you estimate this relative difference? Would you expect these proteins to sediment at different rates in an analytical centrifuge? If so, what would the relative protein sedimentation velocities be?
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To address the question well consider the principles of capillary electrophoresis and sedimentation in a centrifuge Step 1 Understanding Diffusion Coefficients and Electrophoresis Diffusion Coefficien... View full answer
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