Question: The final step in a protein isolation process results in a mixture of protein and ammonium sulfate salt (NH4)2SO4 and you want to remove the
The final step in a protein isolation process results in a mixture of protein and ammonium sulfate salt (NH4)2SO4 and you want to remove the salt before doing further experiments. Note: This salt dissociates in solution into ammonium (NH4+)and sulfate (SO42) ions. To remove this salt you perform dialysis. The mixture has a volume of 10.mL, and is 5.0% ammonium sulfate (MW 132.14g/mol). If you dialyze this 10 . mL sample twice (that is you perform 2 dialysis steps) in a 0.0% ammonium sulfate buffer with a volume of 2.0 L each time, then what will the final salt concentration be in percent (NH4)2SO4 ? Your
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