The following data on bakers' yeast in a particular medium at 23.4C were obtained in the...

 

Transcribed Image Text:

The following data on bakers' yeast in a particular medium at 23.4°C were obtained in the presence and in the absence of an inhibitor, sulfanilamide. The reaction rate (-rS) was measured in terms of the oxygen uptake rate Qo, obtained as a function of oxygen partial pressure. (a) Assume the rate Q, follows Michaelis-Menten kinetics with respect to oxygen. Calculate the Q, maximum (i.e., Vmax), and the Michaelis-Menten constant KM. [Ans.: Vmax = 52.63 µL 02/h • mg cells.] (b) Using the Lineweaver-Burk plot, determine the type of inhibition sulfanilamide that causes the 02 uptake to change. Po Co, (no sulfanilamide) Po, (20 mg sulfanilamide/mL added to medium) 0.0 00.0 00.0 0.5 23.5 17.4 1.0 33.0 25.6 1.5 37.5 30.8 2.5 42.0 36.4 3.5 43.0 39.6 5.0 43.0 40.0 Po, = oxygen partial pressure, mmHg; Co̟= oxygen uptake rate, µL of 02 per hour per mg of cells. (c) List ways you can work this problem incorrectly. The following data on bakers' yeast in a particular medium at 23.4°C were obtained in the presence and in the absence of an inhibitor, sulfanilamide. The reaction rate (-rS) was measured in terms of the oxygen uptake rate Qo, obtained as a function of oxygen partial pressure. (a) Assume the rate Q, follows Michaelis-Menten kinetics with respect to oxygen. Calculate the Q, maximum (i.e., Vmax), and the Michaelis-Menten constant KM. [Ans.: Vmax = 52.63 µL 02/h • mg cells.] (b) Using the Lineweaver-Burk plot, determine the type of inhibition sulfanilamide that causes the 02 uptake to change. Po Co, (no sulfanilamide) Po, (20 mg sulfanilamide/mL added to medium) 0.0 00.0 00.0 0.5 23.5 17.4 1.0 33.0 25.6 1.5 37.5 30.8 2.5 42.0 36.4 3.5 43.0 39.6 5.0 43.0 40.0 Po, = oxygen partial pressure, mmHg; Co̟= oxygen uptake rate, µL of 02 per hour per mg of cells. (c) List ways you can work this problem incorrectly.

Answer rating: 100% (QA)

Answer Consider Ckygen op take rete cbeye Michaelis menten kinetics Expres the ... View the full answer