Question: The following data were obtained from 3 separate enzyme kinetic experiments using 3 different substrates S1, S2 and S3 forming products P1, P2 and P3

The following data were obtained from 3 separate enzyme kinetic experiments using 3 different substrates S1, S2 and S3 forming products P1, P2 and P3 respectively. The amount of enzyme in each reaction is 1 µM. Find out the rate and graph the data using a Michaelis-Menton and Lineweaver-Burk plots and determine the values for K_m, V_max, K_cat, and K_cat/K_m. Which among the 3 substrate is best substrate for this enzyme and why?

[S] (mM)	[P1] (mM) in 60 min	[P2] (mM) in 240 min	[P3] (mM) in 30 min
2.5	0.170	0.126	0.194
2	0.201	0.135	0.213
1.5	0.167	0.129	0.206
1	0.162	0.131	0.203
0.6	0.159	0.135	0.174
0.3	0.148	0.131	0.163
0.15	0.122	0.111	0.122
0.2	0.095	0.117	0.114
0.1	0.075	0.072	0.104
0.05	0.049	0.040	0.071

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