The isolation of a short polypeptide yields 230 kg of butanol extract containing 0.081 wt% peptide. We
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The isolation of a short polypeptide yields 230 kg of butanol extract containing 0.081 wt% peptide. We want to strip this extract with initially peptide-free saline solution at pH 9.0 to yield a product solution containing 0.2 wt% peptide. The equilibrium under these conditions is
y = 0.16x
Where y = mass fraction in butanol, and x = mass fraction in water. We want to recover 98% of this peptide.
How many countercurrent stages do we need for this extraction?
Related Book For
Fundamentals of Analytical Chemistry
ISBN: 978-0495558286
9th edition
Authors: Douglas A. Skoog, Donald M. West, F. James Holler, Stanley R. Crouch
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