The model we will use for examining the research questions is model (1B), Actti = 0 + 1 Treat2ti + 2 Reg2ti + 3 Reg3ti + 4 Treat2ti Reg2ti + 5 Treat2ti Reg3ti +0i +1i Treat2ti +ti. The expected value of Actti in model (1B) is referred to as the marginal value of Actti , and is given by E(Actti)=0+1Treat2ti +2Reg2ti +3Reg3ti +4Treat2ti Reg2ti +5Treat2ti Reg3ti. The marginal value of Actti for rats who are administered the Basal treatment and are measured in the BST brain region is E(Actti|Treat = Basal,Reg = BST) = 0. Treat = Basal means that Treat2i = 0, and Reg = BST means that Reg2ti = 0 and Reg3ti = 0.The expected value of Actti in model (1B), conditional on the child-specific random intercept and random effect of Treat2, is referred to as the conditional value of Actti , and is given by E(Actti|0i,1i)=0 +1Treat2ti +2Reg2ti +3Reg3ti + 4 Treat2ti Reg2ti + 5 Treat2ti Reg3ti +0i +1i Treat2ti. Each child has their own conditional value of Actti .Let 0, 1, 2, 3, 4 and 5 denote the GLS estimates of the fixed effects 0, 1, 2, 3, 4 and 5, respectively. The predicted marginal value of Actti is given by E(Actti)=0+1Treat2ti +2Reg2ti +3Reg3ti +4Treat2ti Reg2ti +5Treat2ti Reg3ti. A marginal residual for model (1B) is the difference between the observed value of Act and the predicted marginal value of Act and is given by =Act E(Act ).What is the correct interpretation of 32 in model (1B) ?

Question 4 Select one:

a. 32 is the difference in mean nucleotide activation between rats measured in the VDB brain region and rats measured in the LS brain region, where the rats are administered the Basal treatment.

b. 32 is the difference in mean nucleotide activation between rats measured in the VDB brain region and rats measured in the LS brain region, where the rats are administered the Carbachol treatment.

c. 32 is the mean nucleotide activation for rats who are administered the Basal treatment and are measured in the LS brain region.

d. 32 is the mean nucleotide activation for rats who are measured in the LS brain region and the BST brain region.