This is all part of one question. Please explain steps.. Thank you!! :)

Running buffer: Refer to the Western Blot Solution handout: a. How many grams of Tris-base do you need to weigh in order to make 500 ml of 10x Running buffer? b. How many grams of glycine do you need to weigh in order to make 500 mls of 10X Running buffer? c. You will run your gel using 1X Running Buffer. What dilution do you need to make prior to running your gel? You need a final volume of 1L d. How would you make a 10% SDS? According to the recipe the 1xRB has to have a final concentration of 0.1% SDS? How much of your 10% stock solution would add to your 1x RB buffer (1L final volume)? e. To finish your 1RB how much dH2O would you add to make 1L ? How many grams of each component would you need in order to prepare 1L of Transfer Buffer (recipe is in the handout): How much MeOH do you need to add for a final concentration of 20% ? Following the recipe in the handout for PBS how many grams of each component do you need to weigh in order to make 1L