To assemble a genetic map of corn plants based on four recessive mutant loci: yellow-green seedling (yg),
Question:
To assemble a genetic map of corn plants based on four recessive mutant loci: yellow-green seedling (yg), virescent (v), purple aleurone (pr), and brittle stalk (bs), here is the experiment design: Cross two true breeding corn plants that are either completely wild-type or are mutant for all four loci and get F1 wild type plants. Then the F1 tree was manually self-crossed to obtain F2 seeds. You plant the F2 seeds from F1 self-crossing for a corn of resultant F2 plants. Additionally, It has been known these four loci located at two different chromosomes. Based on the following frequencies of mutant classes:
(1) Determine which loci are likely to be on the same chromosome and which locus (loci) appear to segregate independently
(2) For the linked loci, calculate the mapping distance and draw the genetic linkage map. You need to show the detailed analysis to make the conclusion. (attention: you do not need to consider the situation in which the parental phenotype in F2 population were derived from the formation of two recombinant gametes since the chance of such parental F2 plants is very low)
Phenotype | # of plants | Phenotype | # of plants |
Normal | 402 | yellow-green seedling and purple aleurone | 37 |
brittle stalk | 127 | yellow-green seedling | 18 |
purple aleurone | 115 | virescent, purple aleurone, and brittle stalk | 12 |
yellow-green seedling and virescent | 108 | virescent | 11 |
virescent and purple aleurone | 54 | yellow-green seedling and brittle stalk | 10 |
yellow-green seedling, virescent, and brittle stalk | 42 | yellow-green seedling, virescent, purple aleurone, and brittle stalk | 8 |
yellow-green seedling, virescent, and purple aleurone | 41 | virescent and brittle stalk | 3 |
purple aleurone and brittle stalk | 38 | yellow-green seedling, purple aleurone, and brittle stalk | 2 |