Question: Two samples were obtained and submitted for testing. The first sample was found in the back-passenger seat of Mr. Alexander Rios (1A) on the lower

Two samples were obtained and submitted for testing. The first sample was found in the back-passenger seat of Mr. Alexander Rios (1A) on the lower right corner of the seat cushion. A second sample (2A) was collected on the same seat, on the cushion. Various techniques were performed in order to determine the content of the samples found. These included: Phenolphthalein, Acid Phosphatase, Hematrace cards, Polymerase Chain Reaction, and Genotyping using STR results from a Qiagen Identifiler Kit. A summary of the techniques, and their listed results is found below: Phenolphthalein o Analysis Acid Phosphatase o Analysis Hematrace o Analysis

Item Blood Semen Saliva Other
1A Pos. (Phenolphthalein) Neg. (Acid Phosphatase) NT INC (Hematrace)
2A Pos. (Phenolphthalein) Neg. (Acid Phosphatase) NT Pos. (Hematrace)

Pos - Positive; Neg - Negative; Inc - Inconclusive; NT - Not tested

Genotypes CSF1PO D10S1248 D12S391 D13S317 D16S539 D18S51 D19S433 D1S1656 D21S11 D22S1045 D2S1338
1A 10 10 16 17 15 17 11 13 12 12 12 14 28 33.2 11 12 20 25
2A 10 12 15 19 11 14 11 12 12 19 14 15 12 18 28 29 16 25

Genotypes D2S441 D3S1358 D5S818 D7S820 D8S1179 FGA TH01 TPOX vWA Amel
1A 14 16 14 15 9 12 6 9.3 11 11 15 16 X X
2A 12.3 12.3 15 17 9 12 8 10 14 14 19 26 6 9 8 8 16 17 X Y

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