Using D$-$glyceraldehyde$-3-$phosphate as substrate and an enzyme concentration of $2\mathrm{.}22$ x

$10-10$ M$,$ the following data were obtained for the initial velocity $($v$)$ for the forward

reaction:

$[$S$]($mM$)0\mathrm{.}140\mathrm{.}290\mathrm{.}450\mathrm{.}621\mathrm{.}202\mathrm{.}94$

V$0($mM s$-1$

$)2\mathrm{.}624\mathrm{.}906\mathrm{.}747\mathrm{.}8011\mathrm{.}314\mathrm{.}9$

$($i$)$ Determine the Michaelis$-$Menten constant

$5$ marks

$($ii$)$ If a different substrate was used for this enzyme and higher value for the MichealisMenten constant was determined, what would this tell you about how the enzyme

and this different substrate interact compared to how D$-$glyceraldehyde$-3-$

phosphate interacts with the enzyme?

$3$ marks

$($iii$)$ Determine kb$,$ the rate constant for the breakdown of the enzyme substrate

intermediate to form product.