19. Read the following steps carefully and complete the exercises with proper analytical rationale. A. TWO...

 

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19. Read the following steps carefully and complete the exercises with proper analytical rationale. A. TWO sets of asynchronous cells with the following populations are plated in different plates: Cell count G1-300; G2-300 $-300; 5-8 hours M-800 G2-4 hours M-1 hour Time Intervals 61-11 hours B. The plates had a PE of 100% and they were incubated for an additional 48 hours C. After 48 hours, one of the plates (Q) was exposed to continuous X-ray LDR radiation (0.35Gy/h) for 24 hours while hydroxyurea was added to the second plate (R) left for 24 hours. D. Plate Q: the LDR exposure was stopped and the plate was incubated for an additional 24 hours. BrdU was added after the 24-hour incubation; BrdU is present in all subsequent steps. E. Plate R: hydroxyurea was removed and incubated for an additional 24 hours. BrdU was added after the 24-hour incubation and BrdU is present in all subsequent steps. F. Both Plates: 24 hours after the initial introduction of Brdu, both cell plates counted and probed for fluorescence G. Both plates were exposed to the same dose of 3 Gy with the same dose rate and aeration. They were incubated in the same environment. The cells were counted again. Exercise: a. Estimate the number of cells in each phase after step B for each plate Plate Q G1= Plate R G1= G2= M= G2= M= b. Estimate the number of cells in each phase just before steps D and E Step D Plate Q Plate R Step E Plate Q Plate R G1= G1= bű G1= G1= nu G2= G2- G2- G2= TOTAL = TOTAL = Ma Ma M M= d. Estimate how many cells in each plate would exhibit fluorescence Plate Q Plate R I TOTAL = TOTAL = TOTAL = TOTAL = 4 points TOTAL TOTAL = c. Qualitatively assess the number of cells in each phase after steps G 1 point Based on all of the data given above, combined with the calculated numbers along with the theory, which of the plates will have a higher survival fraction? 4 points 1 point 19. Read the following steps carefully and complete the exercises with proper analytical rationale. A. TWO sets of asynchronous cells with the following populations are plated in different plates: Cell count G1-300; G2-300 $-300; 5-8 hours M-800 G2-4 hours M-1 hour Time Intervals 61-11 hours B. The plates had a PE of 100% and they were incubated for an additional 48 hours C. After 48 hours, one of the plates (Q) was exposed to continuous X-ray LDR radiation (0.35Gy/h) for 24 hours while hydroxyurea was added to the second plate (R) left for 24 hours. D. Plate Q: the LDR exposure was stopped and the plate was incubated for an additional 24 hours. BrdU was added after the 24-hour incubation; BrdU is present in all subsequent steps. E. Plate R: hydroxyurea was removed and incubated for an additional 24 hours. BrdU was added after the 24-hour incubation and BrdU is present in all subsequent steps. F. Both Plates: 24 hours after the initial introduction of Brdu, both cell plates counted and probed for fluorescence G. Both plates were exposed to the same dose of 3 Gy with the same dose rate and aeration. They were incubated in the same environment. The cells were counted again. Exercise: a. Estimate the number of cells in each phase after step B for each plate Plate Q G1= Plate R G1= G2= M= G2= M= b. Estimate the number of cells in each phase just before steps D and E Step D Plate Q Plate R Step E Plate Q Plate R G1= G1= bű G1= G1= nu G2= G2- G2- G2= TOTAL = TOTAL = Ma Ma M M= d. Estimate how many cells in each plate would exhibit fluorescence Plate Q Plate R I TOTAL = TOTAL = TOTAL = TOTAL = 4 points TOTAL TOTAL = c. Qualitatively assess the number of cells in each phase after steps G 1 point Based on all of the data given above, combined with the calculated numbers along with the theory, which of the plates will have a higher survival fraction? 4 points 1 point

Answer rating: 100% (QA)

Solution a To start it is needed to understand that cell division only happen during the M phase In ... View the full answer