Using the equations (discussed above) showing the relationship between (acid], [conjugate base), and [buffer concentration), mathematically show the negligible change in pH even when 2 uL of 10M HCl is added to 100 mL of 50 mM phosphate buffer at pH 6.9. To address this problem, the following steps will be needed: (i) You will have to calculate the pH of the buffer after adding the 2 uL of 10M HCl (ii) To do this, calculate the new concentration of [acid) and (conjugate base) after the addition of 2 uLof 10M HCl, and (iii) To appreciate the effect of buffers, show the pH change in 100 mL of water after adding 2 uL of 10M HCl that does not contain the phosphate buffer ions. (Show all calculation steps) Points 15 (H-H equation: 1 + calculation of (acid]: 4 + calculation of (conjugate base): 4 + calculation of new pH: 3 + calculation of pH of water upon addition of HCl: 3) If the concentration of buffer in problem no. 2 were 250 mM phosphate, what would the change in pH be? Points 5 (calculation of (acid]: 2 + calculation of (conjugate base]: 2 + calculation of new pH: 1]