Question: Using the fed-batch data in the excel spreadsheet provided, calculate (a) cell specific growth rate and doubling time, (b) cell specific glucose consumption and lactate
Using the fed-batch data in the excel spreadsheet provided, calculate (a) cell specific growth rate and doubling time, (b) cell specific glucose consumption and lactate production rates, and (c) the cell specific IgG production rates. Calculate these parameters using differential and integral methods. For (a) and (b) focus only on the exponential phase of the growth while for (c) perform the analysis for the whole culture period.
| Days in Culture | Viable cell density | Viability | Nova pH | Nova Glucose (Gluc) g/L | Nova Lactate (Lac) g/L | Titer (mg/L) | |
| 0.0 | 1.87E+05 | 90.30 | 7.201 | 6.1 | 0 | 0 | |
| 1.4 | 3.86E+05 | 95.20 | 7.042 | 6 | 0.05 | 18.05 | |
| 2.3 | 6.40E+05 | 93.90 | 7.074 | 5.8 | 0.1 | 29.55 | |
| 2.7 | 8.00E+05 | 93.80 | 7.064 | 5.5 | 0.3 | 36.65 | |
| 3.3 | 1.15E+06 | 93.60 | 6.916 | 5.2 | 0.5 | 58.5 | |
| 3.9 | 1.73E+06 | 93.60 | 6.639 | 4.75 | 0.8 | 65 | |
| 4.5 | 2.40E+06 | 88.80 | 6.688 | 4.1 | 1.1 | 70.5 | |
| 5.0 | 3.76E+06 | 92.40 | 6.698 | 3.1 | 1.8 | 135.5 | |
| 5.7 | 4.94E+06 | 93.20 | 6.683 | 2 | 2.8 | 189.5 | |
| 6.2 | 6.70E+06 | 92.10 | 6.666 | 0.5 | 3.89 | 250.5 | |
| 6.9 | 8.09E+06 | 91.60 | 6.692 | 0 | 4.1 | 368.5 | |
| 7.4 | 8.86E+06 | 88.70 | 6.743 | 0 | 4.05 | 495 | |
| 8.1 | 1.02E+07 | 87.70 | 6.73 | 0 | 4 | 641.5 | |
| 8.6 | 1.17E+07 | 87.00 | 6.75 | 0 | 3.8 | 767.6 | |
| 9.5 | 1.23E+07 | 84.60 | 6.885 | 0 | 3.76 | 962.5 | |
| 9.9 | 1.24E+07 | 81.80 | 6.81 | 0 | 3.7 | 1148 | |
| 10.6 | 1.35E+07 | 79.10 | 6.851 | 0 | 3.44 | 1366 | |
| 11.1 | 1.31E+07 | 76.10 | 6.851 | 0 | 3.4 | 1575 | |
| 11.8 | 1.27E+07 | 72.70 | 6.964 | 0 | 3.2 | 1756 | |
| 12.3 | 1.17E+07 | 63.70 | 6.919 | 0 | 3.4 | 1962 | |
| 12.9 | 9.84E+06 | 59.20 | 6.961 | 0 | 3.2 | 2112 | |
| 13.4 | 8.87E+06 | 47.90 | 6.985 | 0 | 3.12 | 2215 | |
| 14.1 | 7.15E+06 | 40.80 | 7.044 | 0 | 3 | 2328 | |
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