We performed a Western blot on C2012 cell lysates using an antibody against CRTH2. We expect a single band that runs where the arrow points in the developed blot below. We also observe a significant number of other bands that do not correspond to a known CRTH2 isoform. What are the potential sources of these additional bands? How can we clean up this blot to eliminate the additional bands? Change as many parameters as you consider helpful and provide reasons for your suggestions. What controls could be added to this experiment to further confirm that the expected band is indeed CRTH2? What controls could be added to determine the source or identity of the additional bands? Here are the conditions we used in our Western blot method: 50ug C2C12 cell protein lysate per lane 10% polyacrylamide gel . Transferred to PVDF membrane Blocked with 1% BSA for an hour at 4C Primary antibody against CRTH2 1:100 dilution overnight at room temperature Secondary antibody 1:20,000 dilution for 1 hour at room temperature Tacit Screening Quiz v1.0 TACIT THERAPEUTICS Tacit Research Associate Molecular Biology Skills Quiz CRTHE