Question: We will be using a Pierce BCA protein assay kit to determine the protein concentration in non-fat dry milk. First, you will be preparing

We will be using a Pierce BCA protein assay kit to determine

We will be using a Pierce BCA protein assay kit to determine the protein concentration in non-fat dry milk. First, you will be preparing the standard solutions using 2 mg/mL Bovine Serum Albumin (BSA) solution. You are only allowed to use 250 l stock solution (2 mg/ml BSA solution) and P-1000 (100~1000 L) mechanical pipette to prepare standard solutions. Please complete the table of standards and provide the sample calculation. DD water (L) BSA stock soln. (L) Total ( 250 L) BSA conc. (g/mL) 1 0 (blank) 2 125 3 250 4 500 5 750 Sample calculation:

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Mechanical Engineering Questions!

Use the following information for the Quick Study below. (Algo) Miami Solar manufactures solar panels for industrial use. The company budgets production of 4,900 units (solar panels) in July and...

CANMNMM January of this year. (a) Each item will be held in a record. Describe all the data structures that must refer to these records to implement the required functionality. Describe all the...

As the XOR operation is not defined on the set {A, . . . , Z}, we replace it here during encryption with modulo-26 addition (e.g., C D = F and Y C = A). (i) Decrypt the following ciphertexts, which...

Use Eq. (6) to estimate Af = f(3.53, 8.98) - f(3.5,9)

Smoothing the Business Cycle versus Fostering Economic Growth: Psychology Meets Normative Macroeconomics: It is sometimes argued that there is a policy trade-off between softening the impact of...

In this simulation, you are asked to address questions regarding the FASB conceptual framework. Prepare responses to allparts. KWW Professlonal Simulation Conceptual Framework Time Remaining 4 hours...

When Carol visits the local supermarket she buys a pack of crisps with probability 0.3, a chocolate with probability 0.4 and her favorite fruit juice with probability 0.6. The probability that she...

On January 1, 2021, the general ledger of Big Blast Fireworks includes the following account balances: The $30,000 beginning balance of inventory consists of 300 units, each costing $100. During...

A corporation reports the following year-end balance sheet data. The company's current ratio equals: nt Cash Accounts receivable Inventory Equipment Total assets $ 49,000 Current liabilities 64,000...

Identify the following users as either External users or Internal users. a. Customers b. Suppliers c. External auditors d. Business press e. Managers f. District attorney g. Shareholders h. Lenders...

Check m Guerra Electronics manufactures a variety of electronic gadgets for use in the home. Which of the following would probably be the most accurate measure of activity to use for allocating the...

Aguila Corporation produces a single product. Variable manufacturing overhead is applied to products on the basis of direct labor-hours. The standard costs for one unit of product are as follows:...

Schedule of Cost of Goods Manufactured and Sold At December 31, 2019, the end of its fiscal year, Lederman Manufacturing Corporation collected the following data for 2019: BusinessCourse Return to...

Matching: The impact of recycling The impact of city-wide recycling and composting initiatives in major Canadian cities The impact of city-wide composting programs in South Vancouver to 1. Too narrow...

Finally, come up with a procedure for assigning a single number "best estimate" and an associated "uncertainty" for each case. By procedure we mean a formula (or else at least an algorithm) which...

Problem 9.07 The water tank has a rectangular crack on its side having a width of 100 mm and an average opening of 0.1 mm Laminar flow occurs through the crack. The water is at a temperature of...

If not profits, then what should a CFO chase? Justify your answer with 3 important weaknesses with the choice of Profits.

Write an SQL statement to display all data on products having a QuantityOnHand greater than 0.

Recently chemical engineers have become involved in the area knows as waste minimization. This involves the operation of a chemical plant so that impacts on the environment are minimized. Suppose a...

Evaluate the integral of the following tabular data with (a) The trapezoidal rule and (b) Simpsons rules: -2 0 2 6 8 10 35 5 -10 2 5 3 20 f(x)

The volume V of liquid in a hollow horizontal cylinder of radius r and length L is related to the liquid h by Determine h given r = 2m, L = 5m, and V = 8.5 m3. Note that if you are using a...

57 Fighting probability of fallow deer bucks. In Aggressive Behavior (Jan./Feb. 2007), zoologists investigated the likelihood of fallow deer bucks fighting during the mating season. During a 270-hour...

56 Abortion provider survey. The Alan Guttmacher Institute Abortion Provider Survey is a survey of all 238 known nonhospital abortion providers in the United States ( Perspectives on Sexual and...

51 Toxic chemical incidents. Process Safety Progress (Sept. 2004) reported on an emergency response system for incidents involving toxic chemicals in Taiwan. The system has logged over 250 incidents...