Question: What does adding salt do during ion-exchange chromatography? It denatures proteins to release (elute) them from the beads in the column. It competes with proteins
What does adding salt do during ion-exchange chromatography? It denatures proteins to release (elute) them from the beads in the column. It competes with proteins for binding to the charged beads. This releases (elutes) them from the column. It helps proteins bind to the beads in the column. It gives all proteins a negative charge for electrophoresis
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