After purifying tyrosinase, you wish to know how much total protein you have purified You use...
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After purifying tyrosinase, you wish to know how much total protein you have purified You use a Bradford protein assay to determine your protein concentration. Your sample containing tyrosinase has an absorbance reading of 0.655. You use BSA as your standard and receive the following data: BSA amount (ug) Absorbance at 595nm 0.5 0.205 1.0 0.410 2.0 0.805 4.0 1.600 а. Draw your standard curve below. Don't forget to label your axes. 1.4 1.2 1.0 0.8 0.u 0.4 0.2 0.5 1.0 2.0 4.0 prokin (ag) b. What is the concentration of your sample if you used 25 uL? What is the specific activity of your enzyme if the relative activity is 45 U/uL? C. ro After purifying tyrosinase, you wish to know how much total protein you have purified You use a Bradford protein assay to determine your protein concentration. Your sample containing tyrosinase has an absorbance reading of 0.655. You use BSA as your standard and receive the following data: BSA amount (ug) Absorbance at 595nm 0.5 0.205 1.0 0.410 2.0 0.805 4.0 1.600 а. Draw your standard curve below. Don't forget to label your axes. 1.4 1.2 1.0 0.8 0.u 0.4 0.2 0.5 1.0 2.0 4.0 prokin (ag) b. What is the concentration of your sample if you used 25 uL? What is the specific activity of your enzyme if the relative activity is 45 U/uL? C. ro
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Chemistry The Central Science
ISBN: 978-0321696724
12th edition
Authors: Theodore Brown, Eugene LeMay, Bruce Bursten, Catherine Murphy, Patrick Woodward
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