What is the correct order of steps you will need to under take in the laboratory to
Question:
What is the correct order of steps you will need to under take in the laboratory to achieve this gene cloning task?
a. 1. Cut genomic DNA and vector DNA with a restriction enzyme(s).
2. Grow E. coli cells on selective medium to select for transformants.
3. Ligate genomic DNA and vector DNA together.
4. Transform E. coli cells with recombinant DNA molecules.
5. Hybridize clones with a labelled probe. 6. Select a clone that hybridizes to the probe.
b. 1. Cut genomic DNA and vector DNA with a restriction enzyme(s).
2. Ligate genomic DNA and vector DNA together.
3. Transform E. coli cells with recombinant DNA molecules.
4. Grow E. coli cells on selective medium to select for transformants.
5. Hybridize clones with a labelled probe.
6. Select a clone that hybridizes to the probe.
c. 1.Cut genomic DNA and vector DNA with a restriction enzyme(s).
2. Ligate genomic DNA and vector DNA together.
3. Grow E. coli cells on selective medium to select for transformants
4. Transform E. coli cells with recombinant DNA molecules.
5. Hybridize clones with a labelled probe. 6. Select a clone that hybridizes to the probe.
d. 1. Cut genomic DNA and vector DNA with a restriction enzyme(s).
2. Transform E. coli cells with recombinant DNA molecules.
3. Ligate genomic DNA and vector DNA together.
4. Grow E. coli cells on selective medium to select for transformants.
5. Hybridize clones with a labelled probe.
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