Question: You will need to understand solved problem S4 before answering this question .A gene that is normally expressed in pancreatic cell was cloned and then

You will need to understand solved problem S4 before answering this question .A gene that is normally expressed in pancreatic cell was cloned and then subjected to promoter bashing. As shown here, four regions, labeled A - D, were individually deleted, and then the DNA was transformed into pancreatic cells or into kidney cells.

The data shown in the following table are from this experiment (see upper right column).

You will need to understand solved problem S4 before answering

If we assume that the upstream region has one silencer and one enhancer, answer the following questions:
A. Where are the silencer and enhancer located?
B. Why don't we detect the presence of the silencer in the pancreatic cells?
C. why isn't this gene normally expressed in kidney cells?

IAI BC D Upstream region Core promoter lacZ gene coding sequence Cell Type Percentage of Region Deleted Transformed B-Galactosidase Activity 100 Pancreatic Pancreatic Pancreatic Pancreatic Pancreatic Kidney Kidney Kidney Kidney Kidney None 100 100

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