What type of FACS data graphical representations are Panel A and Panel B? (1 Mark) All data displayed in Panel B have been gated from data displayed in Panel A using a FSC and SSC gate. What does this statement mean? Why would you want to do this? (1 Mark) Which of the shRNA vectors targeted to Scribble (1 or 2) is most effective in knocking down Scribble? Is it as effective as the shRNA targeting GFP? Why might this be the case? (1 Mark) Does loss of expression of Scribble in the above experiment alter cell shape or cell size? Explain your rationale for the answer. (1 Mark) If the various transfected cell populations above were sorted using the FSC and SSC gate and plated onto a glass slide, and examined by fluorescence microscopy for GFP, what would be the main difference you would observe between the shGFP alone and the shScribl transfected cells in terms of GFP intensity? What chemical stain might you use to identify the nucleus of each cell? How does it work?

2% Ims.latrobe.edu.au/mod/quiz/attempt.php?attempt=16137209cmid=7753334page=2 Reload this page to apply your updated settings on this site Reload X My LMS subjects Question 3 To examine the function of the Scribble polarity gene, two shRNA sequences (shScrib1 and shScrib2) Not yet 1 2 4 5 answered have been designed to knockdown Scribble expression. To test their effectiveness, along with a negative control (empty vector) and a positive control (shGFP) (which can also knockdown the Marked out of Finish attempt ... 5.00 Scribble-GFP fusion), they have been tested on HEK293 cells that stably express Scribble GFP. In Panel B, Blue represents untransfected cells, Red represents transfected cells. Flag question A B HEK293-Scribble-GFP cells Empty vector transfection HEK293-Scribble-GFP cells shGFP vector transfection SSC Cell number HEK293-Scribble-GFP cells shScrib1 vector transfection HEK293-Scribble-GFP cells shScrib2 vector transfection 260 20 680 650 1600 FSC GFP Intensity A. What type of FACS data graphical representations are Panel A and Panel B? (1 Mark)