Question: When you are doing your calculations - remember that the initial glucose concentration was 0, 0.1, 0.3... etc ... but then - in step 3.3
When you are doing your calculations - remember that the initial glucose concentration was 0, 0.1, 0.3... etc ... but then - in step 3.3 you've added more solvent (reagent and water) ... so your concentrations change by about two decimal points in mM. It might be helpful to create a large table with the sample information, absorbance, initial [Glu], diluted [Glu], expected [Glu] before hydrolysis (does it reduce or not? and into how many 'pieces') ... and then the measured [Glu]. Using the measured [Glu] / expected [Glu] you will get % hydrolysis (which will be less than 100%
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