With reference to the paper by Win & Smolke, propose (draw and annotate) a ribo-regulated gene network that realizes a band-pass filter, where 'band' here actually means concentration of some input molecule. The network should generate GFP only when the concentration of the input molecule falls between a lower and an upper bound. Hint: it is possible to use proteins (and non-coding RNA molecules) as inputs to riboswicthes. The main figure of the article is provided below. Thanks!

A Coupled gate device B input A input A 24. [SI 1.1] 17.5 -178 -186 15. Device signal (%%) Device response in unit expression 9. 8.3 AAAAA 2xBuffera O 2xBuffers Buffory Buffer t 2xButler1 Buttere Buffer-to Butfore Buffer-4c Inverter! Inverter# Inverter4 2xbutfor 1 2xinwortor1 Inverter 2.Butter-ic 2xInverter4 2xinverter 2inverter4 Buter1 +Buffers Buffer1 + Butters Inverter 1+Inverters[ Output protein (high when AA) C D AND gate 16 14.0 input A input B AB output [SI 1.2] B output theo to GFP 8 . Device response in unit expression 6 - 3.1 2.0 T GFP low low low high theo + + Output protein (high when AB) to E NOR gate F 10 input A input B 8.1 A+B - output [SI 1.3] B output theo to GFP Device response in unit expression 1 3- 2.0 AAAAA 2 - T 1.1 0 GFP high low low low theo + Output protein (high when A+B) to - - + + Fig. 2. RNA devices based on signal integration within the 3' UTR (SI 1). the Inverter gates. (C) An RNA device that performs an AND operation by Single-input gates are indicated in dashed boxes, and triangles indicate coupling two Buffer gates responsive to different inputs and the associated relationships between associated gate inputs and outputs. (A) An RNA device truth table. (D) The device response of an AND gate (L2bulge1 + L2bulgeltc). composed of two Buffer gates responsive to the same input functions to shift Device response under different input conditions [theo or to (-), 0 mM; theo the device response lower than that of the single-input gate. (B) The device (+), 5 mM; tc (+), 0.25 mM] is reported as the difference between expression output of RNA devices composed of two single-input gates and their single- activity in the absence of both inputs and that at the indicated input input gate counterparts. (Left) Device response (bars) is reported as the conditions. (E) An RNA device that performs a NOR operation by coupling difference between gene expression activities in the absence and presence of two Inverter gates responsive to different inputs and the associated truth the appropriate inputs [10 mM theophylline (theo) or 1 mM tetracycline (to)] table. (F) The device response of a NOR gate (L2bulgeOff1 + L2bulgeOff1tc). (21). (Right) Device signal (arrows) is reported over the full transcriptional Device response under different input conditions [theo or to (-), 0 mM; theo range of the promoter system used as a percentage of the expression activity (+), 10 mM; tc (+), 0.5 mM] is reported as the difference between expression relative to that of an inactive ribozyme control, where circles and arrowheads activity in the presence of both inputs and that at the indicated input indicate device signals in the absence and presence of input, respectively. The conditions. Error bars represent the SD from at least three independent negative sign indicates the down-regulation of target gene expression by experiments