1). With your knowledge in a polymerase chain reaction (PCR) and forms of polymorphism, how can you use Simple Sequence Repeat (SSR) to determine the Biological father of a newborn baby?

2). The procrastination gene makes students lazy for studies. Assume the gene is made up of 9 exons and you want to knock out this procrastination gene and replace a thousand hardworking genes, Outline the procedure in creating a knock-out organism.

3). In your haste, you have set up a DNA sequencing reaction with the following reagents: denatured DNA, 4 deoxynucleotide triphosphate (NTPs). DNA polymerase and 32P-labeled primer.

a). What do you think your sequencing per would look like?

b). How can you correct the mistakes time?  

4). Compare and contrast between prokaryotic and eukaryotic cut the molecular level?