1. Write a plain text description of what each line in the program does. You must include a POF description for each line highlighted in bold.
2. Draw a plausible structure for the molecule analysed (chemical formula: C₈H₉NO₂). Provide a complete table of the ¹H and ¹³C chemical shifts for the compound analysed, including figures where appropriate. Provide an assignment strategy.
3. A big source of the artefacts in the spectrum is the lack of carbon decoupling. Write the code necessary to include this decoupling.
4. Provide a brief history into the development of the HSQC. Use primary references where possible.
5. How might you employ the HSQC to analyse ligand binding? Include figures where necessary.

2021 NMR coursework

Here is the simplest pulse program for a functional HSQC:

#include

ze

d11

1 d1

p1

d4

(center (p2) (p4):f2)

d4

(p3):f2 (p1 ph1)

d0

(center (p2))

d0

(p3):f2 (p1)

go=1

30m mc #0 to 2 F1QF(caldel(d0, +in0))

exit

ph1=1