Question: You are a bioprocess engineer working for a major pharmaceutical company on downstream process development. Your supervisor asks that you do some preliminary scaling calculations

You are a bioprocess engineer working for a major pharmaceutical company on downstream process development. Your supervisor asks that you do some preliminary scaling calculations based on isotherm data generated by colleagues in laboratory experiments (figure). Specifically, you should calculate the shock front velocity for a loading concentration of 3.0 g L-1 in 50 mM phosphate buffer. You can expect the column to have a diameter of 0.16 m with an inter-particle porosity of 0.35. The volumetric flow rate will be 4 mL min-1. Also calculate the chromatographic velocity after the shock front for which you know that dq/dc is 0.5. In addition, which bed height should the column have to ensure a typical contact time?
 You are a bioprocess engineer working for a major pharmaceutical company

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