Question: You are performing an isocratic chromatography with two components and elution volumes of 4 8 0 and 5 0 0 ml , respectively. Calculate the
You are performing an isocratic chromatography with two components and elution volumes of and ml respectively. Calculate the number of theoretical plates for good separation, ie R Assume that the figure is the same for both components.
Use the formulas:
RVeVeWW
NVeW
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