Question: You are using amylose resin for your column chromatography, let's think about why amylose is being used. What is interacting with the amylose resin (be

You are using amylose resin for your column chromatography, let's think about why amylose is being used. What is interacting with the amylose resin (be specific)? What type of chromatography is being used (ionexchange, etc.)? Be more specific than "gravity column chromatography". How will you be disrupting this interaction when you no longer want the protein attached to the resin in the column? Why is glycerol added before storing your enzyme in a 20C freezer

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemistry Questions!

Consider a student who has the choice to buy or not buy a textbook for a course. Well model this as a decision problem with one Boolean decision node, B, indicating whether the agent chooses to buy...

1. Before lab, a culture containing the plasmid pMAL-BAP was incubated in the presence of IPTG. Briefly state how each of the following relate to YOUR protein expression and/or purification. a. Lac...

Asking: Pick your four concentrations in the range of 10-75 M and calculate the dilutions youll need to perform from the stock solution to prepare them. This week in lab, we will be performing two...

How to approach this problem? What do I need to do? I was confused. Please help. Given this information: The stationary phase is often some kind of solid medium, like silica gel or a resin. They...

8. Protein purification. As a graduate student, the first protein I purified was RNA polymerase (RNAP) from E. coli. Some physical and chemical properties of E. coll RNAP Molecularmass =470,000g/mol...

PUTEX CORPORATION: CORPORATE PLANNING PROCESS It was early morning of Monday, March 24, 2014, Gupta was driving to his office located on outskirts of the city. His mind was totally occupied with the...

An elephant weighing 1000 kg tries to walk on amorphous polymer A (Tg=100C) at room temperature. If your walking speed is 100-9999 times/s, you will fall into the polymer, and if your walking speed...

One construction method employed in many public buildings is to have the exterior building walls composed of a large number of small tiles. These tiles are generally cemented into place on the...

Question 2 (a) (Aqueous) 10% w/w-acetic acid is produced in a bioprocess reactor. Propose separation sequences (block diagram) that will produce a target acetic acid (boiling point 118 C at 1 atm...

For the Apple prices of Exercise 5, find the lag1 version of the prices. Month Apples Gas Jan Feb Mar Apr May 1021 2.993 Jun Jul Aug 1.235 3.033 Sep 1256 2637 Oct Nov 1.089 2.287 Dec 0.963 2359 0.977...

Donaldson & Son has an ROA of 10%, a 2% profit margin, and a return on equity equal to 15%. What is the companys total assets turnover?What is the firms equity multiplier?

Your degree of risk aversion ( A ) is 2 . The risky asset's expected return is 1 2 . 4 % , while the risk - free rate is 2 . 5 % . The risky asset has a volatility of 1 6 % . You have $ 1 , 0 0 0 to...

DRAW A SCHEMATIC OF YOUR HOME. IDENTIFY THE DEVICES IN EACH ROOM. ASSIGN A PORT TO EACH ROOM. EACH PORT IS A 8-BIT PORT. EACH BIT CAN CONTROL A DEVICE THROUGH A RELAY. INFORMATION FOR TURNING ON/OFF...

Message Strategies: Refusing Routine Requests [LO-4] Read the following document and (a) analyze the strengths and weaknesses of each sentence and (b) revise the message so that it follows this...

Message Strategies: Making Negative Announcements; Communication Ethics: Distinguishing Ethical Dilemmas and Ethical Lapses [LO-4], Chapter 1 The insurance company where you work is planning to raise...

The snack maker you purchased six years ago is obsolete technology. We do not seem to have relevant replacement parts in our inventory. You may check with the dealer from whom you purchased it.