Question: You have to do a restriction digest in 50 ul with 1 ul enzyme, and a buffer that comes at 5X. How many ul of

  • You have to do a restriction digest in 50 ul with 1 ul enzyme, and a buffer that comes at 5X. How many ul of buffer do you use?

  • You have to transfect cells with 20 ng of DNA. You have a solution of 6,000 ng/ul, how many ul do you use?
  • 250 mL of 1M Tris, pH 8: how much volume to bring it up into, knowing you have to pH first?
    • For the above solution, if the pH meter reads 2.0, do you add acid or base?
  • How many moles of DNA do you have in a 2 ul of a 100 uM solution?
  • What's the molarity of a 3% NaCl (w/v) solution?

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