You reported 18ml in your lab notebook for your stage 1 enzyme. In your stage 1 aliquot, your PNPP to PNP assay (0.1ml of stage 1+1.5ml of substrate +1.4ml buffer =3.0ml total volume) showed the following: The extinction coefficient "e" of 18,300M1 is used to convert between Absorbance A and molarity [M] in the test tubes used in class. A=e[M] What is the total activity in units of (micromoles of PNP product) /min? You reported 4ml in your lab notebook for your stage 4 enzyme. In your stage 4 aliquot, your PNPP to PNP assay (0.1ml of stage 4+1.5ml of substrate +1.4ml buffer, 3.0ml total volume) showed the following: The extinction coefficient "e" of 18,300M1 is used to convert between Absorbance A and molarity [M] in the test tubes used in class. A=e[M] What percent of the total activity (umol PNP/min) of Stage 1 (Question 1) remains in Stage 4