Your manager thinks that the lab saves money by making its own stock solutions of protease inhibitors.
Question:
Your manager thinks that the lab saves money by making its own stock solutions of protease inhibitors. But, you think you might save money by buying pre-packaged protease inhibitor tablets. Find out which is the least expensive. How much each strategy would cost yearly, for a lab that breaks 100 mL of cells per week
Making it on your own
Protease type | Specific inhibitor | Working concentration | Cost |
Serine | PMSF (MW: 174) Aprotinin | 1 mM 10 µg/mL | $50/ 250 mg $650/ 100 mg |
Cys & Ser | Leupeptin | 10 µg/mL | $796/ 50 mg |
Cysteine | E-64 (MW: 357) | 1 mM | $474 / 25 mg |
Aspartic Acid | Pepstatin | 2 µg/mL | $718/ 100 mg |
Metallo- | EDTA (MW 292) | 1 mM | $29/ 100 g |
Pre made
ThermoFisher sells Pre-made Protease inhibitor with all the above proteases for $749 for 10mL of 100X solution
Based on the yearly calculations from the "pre-made" and "on your own" strategy which one is least expensive and by how much.
