Question: Determine the field gradient required to separate two polypeptides (D = 5 x 10 -6 cm 2 /s), with respective pI values of 4.2 and

Determine the field gradient required to separate two polypeptides (D =  5 x 10-6 cm2/s), with respective pI values of 4.2 and 4.8, using IEF with a linear field gradient of 0.3/cm. Use a standard criteria for IEF that peak-to-peak separation must be three times the average peak width.

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Solution Equation 78 can be rewritten as F GVA GVLTBM G LC1000 kmol A A S L S L TBM Plugging in the... View full answer

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