The release of protein from a suspension of Saccharomyces crevisiae by disruption in an industrial homogenizer was

Question:

The release of protein from a suspension of Saccharomyces crevisiae by disruption in an industrial homogenizer was investigated by Hetherington. [Trans. Instn. Chem. Engrs., 49, 142 (1971)]. The homogenizer pressure was varied, and it was operated on a recycle basis. The results were correlated by the equation

where R is the amount of protein released (mg/g yeast), Rm is the maximum amount of protein available for release, K is a temperature- dependent dimensional constant, P is pressure in kg_f/cm², N is the number of passes through the homogenizer, and c is a constant. Make a plot of log [R_m= (R_m – R)] versus N with P as a parameter, using the data below, and determine the value of the constants K and c.