Question: Suppose you have just determined the DNA base sequence for an especially strong promoter in Escherichia coli and you are interested in incorporating this sequence

Suppose you have just determined the DNA base sequence for an especially strong promoter in Escherichia coli and you are interested in incorporating this sequence into an expression vector. Describe the steps you would use. What precautions are necessary to be sure that this promoter actually works as expected in its new location?

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