Mitosis Methods And Protocols(1st Edition)

Book details

Book Price $67.89 : In Putting Togetherthissetofmethods,aswellasallthemembersofmylaboratoryforproofreading And Correcting The Chapters And Especially Sarah McClelland Who Read All The Chapters Atleasttwiceatvariousstages. Finally,IwishtothankLyndyRasmusenforherhelpin Administeringtheprojectandkeepingmeorganized. Andrew D. McAinsh V Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . V Contributors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Ix 1 Knock-inandKnock-out:TheUseofReverseGeneticsinSomatic CellstoDissectMitoticPathways . . . . . . . . . . . . . . . . . . . . . . . 1 AlexisR. Barr, DeborahZyssandFanniGergely 2 FunctionalDissectionofMitoticRegulatorsThroughGene TargetinginHumanSomaticCells. . . . . . . . . . . . . . . . . . . . . . . 21 EliBerdougo, Marie-EmilieTerretandPrasadV. Jallepalli 3 RNAiinDrosophilaS2CellsasaToolforStudyingCell CycleProgression. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 Monica ´ Bettencourt-DiasandGohtaGoshima 4 ProductionofMitoticRegulatorsUsinganAutoselectionSystem ForProteinExpressioninBuddingYeast. . . . . . . . . . . . . . . . . . . . 63 MarcoGeymonat, AdonisSpanosandStevenSedgwick 5 HydrodynamicAnalysisofHumanKinetochoreComplexes DuringMitosis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 SarahE. McClellandandAndrewD. McAinsh 6 IsolationofProteinComplexesInvolvedinMitosisandCytokinesis FromDrosophilaCulturedCells. . . . . . . . . . . . . . . . . . . . . . . . . 99 PierPaoloD’Avino,VincentArchambault,MarcinR. Przewloka, WeiZhang,ErnestD. LaueandDavidM. Glover 7 AutomatedLiveMicroscopytoStudyMitoticGeneFunctionin FluorescentReporterCellLines . . . . . . . . . . . . . . . . . . . . . . . . 113 MichaelH. A. SchmitzandDanielW. Gerlich 8 ElectronTomographyofMicrotubuleEnd-Morphologies InC. ElegansEmbryos. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135 EileenO’TooleandThomasMuller-Reicher ¨ T 9 DissectingMitosiswithLaserMicrosurgeryandRNAi InDrosophilaCells. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 145 AntonioJ. Pereira,IrinaMatos,MarianaLince-Faria ´ AndHelderMaiato 10 FluorescenceImagingoftheCentrosomeCycleinMammalianCells . . . . 165 SuzannaL. ProsserandAndrewM. Fry Vii Viii Contents 11 VisualizationofFluorescence-TaggedProteinsinFissionYeast:The AnalysisofMitoticSpindleDynamicsUsingGFP-Tubulin UndertheNativePromoter. . . . . . . . . . . . . . . . . . . . . . . . . . . 185 MasamitsuSato, MikaToyaandTakashiToda 12 AnalysingKinetochoreFunctioninHumanCells:Spindle CheckpointandChromosomeCongression. . . . . . . . . . . . . . . . . . 205 ChristianeKlebig,AlbertoToso,SatyarebalaBorusu AndPatrickMeraldi 13 ProbingKinetochoreStructureandFunctionUsingXenopuslaevis FrogEggExtracts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 221 MichaelJ. EmanueleandP. See more