The half-cell reduction potential is provided by the Nernst equation (Equation 14-8):

(a) On the graph below, plot the reduction potentials for the FADH2/FAD half-cell (E°€² = - 0.219 V) when the [FADH2]/[FAD] ratios are 100, 10, 5, 2, 1, 0.5, 0.2, 0.1, and 0.01 at 25°C versus the percent reduction.

(b) Using the same [Reduced]/[Oxidized] ratios, plot the reduction potentials of cytochrome c (E°€² = 0.235 V).

(c) What is ΔE for the oxidation of FADH2 by cytochrome c when the [FADH2]/[FAD] ratio is 10 and the [cytochrome c (Fe2+)]/[cytochrome c (Fe3+)] ratio is 0.1?

In 12t -0.1 -0.2 -0.3 20 40 60 80 100 % Reduction 0.4 0.3 0.2 0.1 0 20 40 60 80 100 % Reduction