Question: The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows: a. Estimate Vmax and KM

The initial rate for an enzyme-catalyzed reaction has been determined at a number of substrate concentrations. Data are as follows:

a. Estimate Vmax and KM from a direct graph of v versus [S]. Do you find difficulties in getting clear answers?
b. Now use a Lineweaver-Burk plot to analyze the same data. Does this work better?

[S] ( mol/L) v[( mol/L) min 1) 10 20 50 39 65 102 120 135 100 iki 200

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a It is difficult to estimate V max this way A value of about 150 ... View full answer

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