The pH of microscopic vesicles (compartments) in living cells can be estimated by infusing an indicator (HIn)
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A substance that alters color in solution over a constrained range of pH values is known as a pH indicator or acid-base indicator. The indicator chemical just needs to alter color slightly in order to be noticed. Indicators work on the basis that they react with water to produce the hydrogen cation H+ or hydronium ion H3O+. The indicator molecule\\\'s color changes as a result of the reaction. Since indicators have distinct color ranges for color change, they can occasionally be combined to provide color changes over a wider pH range.
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