The study Loss of Nitrogen through Sweat by Preadolescent Boys Consuming Three Levels of Dietary Protein was conducted by the Department of Human Nutrition and Foods at the Virginia Polytechnic Institute and State University to determine perspiration nitrogen loss at, various dietary protein levels. Twelve preadolescent boys ranging in age from 7 years, 8 months to 9 years, 8 months, and judged to be clinically healthy were used in the experiment. Each boy was subjected to one of three controlled diets in which 29, 54, or 84 grams of protein per day were: consumed. The following data represent, the body perspiration nitrogen loss, in milligrams, collected during the last two days of the experimental period:

(a) Perform an analysis of variance at the 0.05 level of significance: to show that the mean perspiration nitrogen losses at the three protein levels arc different.

(b) Use a single' degree-of-freedom contrast with α = 0.05 to compare the mean perspiration nitrogen loss for boys who consume 29 grams of protein per day versus boys who consume 54 and 84 grams of protein.

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Protein Level 54 Grams 8 4 Grams 318 295 271 438 402 29 Grams 390 321 390 190 266 270 399