Question: Two biochemistry students are about to use mitochondria isolated from rat liver for an experiment on oxidative phosphorylation. The directions for the experiment specify addition

Two biochemistry students are about to use mitochondria isolated from rat liver for an experiment on oxidative phosphorylation. The directions for the experiment specify addition of purified cytochrome c from any source to the reaction mixture. Why is the added cytochrome c needed? Why does the source not have to be the same as that of the mitochondria?

Step by Step Solution

★★★★★

3.46 Rating (166 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock

Cytochrome c is not tightly bound to the mitochondri... View full answer

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Document Format (1 attachment)

481-M-S-B-C (418).docx

120 KBs Word File

Students Have Also Explored These Related Biochemistry Questions!

To carefully prepared mitochondria were added succinate, oxidized cytochrome c, ADP, orthophosphate, and sodium cyanide. Referring to Figure 14.16, answer the following. (a) List the sequence of...

Oxidative phosphorylation requires the transfer of electrons donated by NADH. (a) Is NADH imported directly into the mitochondria? Explain. (b) Describe two import mechanisms that transfer cytosolic...

Experiment c is repeated 100 times. Write an integral that estimates the probability that there is an average of less than 1.8 mutants per experiment and shade the corresponding area on a sketch of...

3. [7 marks] A recently opened bottle of wine will smell "off" if sulphur compounds are sufficiently con- centrated. It is of interest to winemakers to determine the "odour threshold", i.e., the...

A recently opened bottle of wine will smell "off" if sulphur compounds are sufficiently con- centrated. It is of interest to winemakers to determine the "odour threshold", i.e., the mini- mum...

A recently opened bottle of wine will smell "off" if sulphur compounds are sufficiently concentrated. It is of interest to winemakers to determine the "odour threshold", i.e., the minimum...

13. Throughout the chapters, the authors make several references to ATP and its "energy" (see case study 2.2 in the book). I feel that you, as biochemistry students, could help me understand a lot...

Please read the questions Question: Please describe your thoughts concerning in this Chapter 5. Key Points 5 . In the United States the number of bilinguals has steadily increased but monolingualism...

Please read the questions Question: What strategies from Chapter 5 do you plan to use with your future students to support their primary languages? Key Points 5 . In the United States the number of...

When might it be particularly appropriate to decrease the size of a product rather than increase its price?

What should auditors do when they have completed an audit and are subsequently asked to report on compliance with regards to certain contractual agreements? a. Accept the engagement and provide...

how to be an optimal enhsnced case manager, take obline courses in project management, to improved organizational skilz

Effect of rent transactions on statement of cash flows A firm reported a balance in its Pre paid Rent (Advances to Landlord) account of $1,200 on January 1, 2009, for use of the building for the...

Performance reports and evaluation Vance Corporation has four divisions: the assembly division, the processing division, the machining division, and the packing division. All four divisions are under...

Four electronic printers are selected from a large lot of damaged printers. Each printer is inspected and classified as containing either a major or a minor defect. Let the random variables X and Y...

Argon gas is approaching a convergingdiverging nozzle with a low velocity at 20C and 120 kPa, and it leaves the nozzle at a supersonic velocity. If the cross-sectional area of the throat is 0.015 m2,...

The isotope: 32/15P is produced by the reaction; n +? 32/15P + p. what must be the target nucleus?

what it says today that it will do tomorrow. Other policies may be credible or noncredible. Which of the following policies are credible? a. A student promises to study for the final after going to...

Given the following peptide SEPIMAPVEYPK a. Estimate the net charge at pH 7 and at pH 12. Assume the pKa values given in Table 5.1. In Table 5.1 b. How many peptides would result if this peptide were...

A mutant form of polypeptide hormone angiotensin II has the amino acid composition The following observations are made: ¢ Trypsin yields a dipeptide containing Asp and Arg, and a hexapeptide...

Can my tutor please explain and answer all of the following question, please show your work Chapter 8.2 3. Revenue of Large Businesses A researcher estimates that the average revenue of the largest...

Just Dew It Corporation reports the following balance sheet information for 2020 and 2021. Based on the balance sheets given for Just Dew it: a. Calculate the current ratio for each year. (Do not...

3. A car dealership offers a lease on an $18000 car. The lessee will pay $2000 down and $200 per month for 36 months. He then has the option to purchase the car for $13000. Instead, he could borrow...