What peptides are expected to be produced when a-melanotropin (Problem 4) is cleaved by a. Trypsin, or
Question:
a. Trypsin, or
b. Cyanogen bromide, or
c. Thermolysin? (Refer to Table 5.3.)
In Table 5.3
Transcribed Image Text:
R, O R. O N-terminal-.-N-C- C N- C-C- C-terminal Enzyme Trypsin Chymotrypsin Thrombin V-8 protease Prolyl endopeptidase R Subtilisin Carboxypeptidase A R2 Thermolysin Cyanogen bromide Preferred Sitea R Lys, Arg R Tyr, Trp, Phe, Leu R1 Arg R1 Asp, Glu Source From digestive systems of animals, many other sources Same as trypsin From blood; involved in coagulation From Staphylococcus aureus Lamb kidney, other tissues From various bacilli Pro Very little specificity C-terminal amino acid From digestive systems of animals R2 Leu, Val, lle, Met From Bacillus thermoproteolyticus
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Related Book For
Biochemistry Concepts and Connections
ISBN: 978-0321839923
1st edition
Authors: Dean R. Appling, Spencer J. Anthony Cahill, Christopher K. Mathews
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