What peptides are expected to be produced when a-melanotropin (Problem 4) is cleaved by
a. Trypsin, or
b. Cyanogen bromide, or
c. Thermolysin? (Refer to Table 5.3.)
In Table 5.3

R, O R. O N-terminal-.-N-C- C N- C-C- C-terminal Enzyme Trypsin Chymotrypsin Thrombin V-8 protease Prolyl endopeptidase R Subtilisin Carboxypeptidase A R2 Thermolysin Cyanogen bromide Preferred Sitea R Lys, Arg R Tyr, Trp, Phe, Leu R1 Arg R1 Asp, Glu Source From digestive systems of animals, many other sources Same as trypsin From blood; involved in coagulation From Staphylococcus aureus Lamb kidney, other tissues From various bacilli Pro Very little specificity C-terminal amino acid From digestive systems of animals R2 Leu, Val, lle, Met From Bacillus thermoproteolyticus