When immunoaffinity chromatography is used to purify a protein, the cell lysate is often subjected to one

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When immunoaffinity chromatography is used to purify a protein, the cell lysate is often subjected to one or more purification steps before the material is applied to the immunoaffinity column. Why is this necessary?

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Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

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Question Posted: Apr 30, 2016 02:28 PM

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When immunoaffinity chromatography is used to purify a protein, the cell lysate is often subjected to one

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Some prepurification before immunoaffinity chromatography may be necessary b...View the full answer

Fundamentals of biochemistry Life at the Molecular Level

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