Why is it necessary to use only the Klenow fragment, rather than intact E. coli Pol I,

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Why is it necessary to use only the Klenow fragment, rather than intact E. coli Pol I, in DNA sequencing reactions (Section 3-4C)?

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Fundamentals of biochemistry Life at the Molecular Level

ISBN: 978-0470547847

4th edition

Authors: Donald Voet, Judith G. Voet, Charlotte W. Pratt

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Question Posted: Apr 30, 2016 02:55 PM

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Why is it necessary to use only the Klenow fragment, rather than intact E. coli Pol I,

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The Klenow fragment which lacks 5 3 exonuclease act...View the full answer

Fundamentals of biochemistry Life at the Molecular Level

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