You have a polypeptide that has been degraded with cyanogen bromide and trypsin. The sequences of the fragments are listed below (using one-letter codes). Is there enough information to derive the complete amino acid sequence of the polypeptide? What further treatments might support the deduced sequence?
Cyanogen bromide treatment: Trypsin treatment:
(1) K
(2) RFM
(3) MLYCRGM
(4) NIKGLM
(5) FMK
(6) GMNIK
(7) GLMR
(8) MLYCR