Question: You have isolated a temperature-sensitive mutant E. coli. At 34C, the growth rate is nearly normal but is reduced 10-fold at 37C. You eventually discover

You have isolated a temperature-sensitive mutant E. coli. At 34°C, the growth rate is nearly normal but is reduced 10-fold at 37°C. You eventually discover that EF-Tu·GDP binding to the ribosome is two-fold higher in the mutant at 37°C. What aspect of translation is affected by the mutation?

Step by Step Solution

★★★★★

3.53 Rating (170 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock

EF Tu GDP dissociation is essential for polypeptide elongation Hence in ... View full answer

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Document Format (1 attachment)

930-M-S-B-C (1790).docx

120 KBs Word File

Students Have Also Explored These Related Biochemistry Questions!

Two mutant strains of E. coli each have a temperature sensitive mutation in a gene that encodes a product required for chromosome duplication. Both strains replicate their DNA and divide normally at...

You have isolated a strain of E. coli that is resistant to penicillin, streptomycin, chloramphenicol, and tetracycline. You also observe that when you mix this strain with cells of E. coli that are...

Signal-transducing trimeric G proteins consist of three subunits designated , and ' The G a subunit is a GTP ase switch protein that cycles between active and inactive states depending on whether it...

4 . You have isolated a mutant strain of S . cerevisiae with a temperature - sensitive phenotype. At the permissive temperature, the mutant cells grow just as well as wild - type cells. At the...

You have isolated a mutant strain of S . cerevisiae with a temperature - sensitive phenotype. At the permissive temperature, the mutant cells grow just as well as wild - type cells. At the...

. You have isolated a mutant strain of S . cerevisiae with a temperature - sensitive phenotype. At the permissive temperature, the mutant cells grow just as well as wild - type cells. At the...

Differentiating Customer Service According to Customer Value at United Parcel Service (UPS) UPS, headquartered in Atlanta, Georgia, is one of the worlds largest package delivery companies....

minicase 6.1 differentiating customer service according to customer value at united parcel service (ups) ups, headquartered in atlanta, georgia, is one of the worlds largest package delivery...

Temperature-sensitive yeast mutants have been isolated that block each of the enzymatic steps in the synthesis of the dolichol-oligosaccharide precursor for N-linked glycosylation. Propose an...

During the first quarter, Roland Company incurs the following direct labor costs: January $40,000, February $30,000, and March $50,000. For each month, prepare the entry to assign overhead to...

solve integral(3(pi), 0) (sin2x-cos4x) dx.

What is the NPV if the discount rate is 1 4 % ? 3 8 , 0 1 5 . 4 2 3 9 , 8 9 3 . 8 3 4 1 , 8 3 7 . 9 7 3 7 , 4 0 9 . 3 3 4 0 , 2 1 8 . 9 0

Continuation of Exercise 3-81. Recall that it was determined that a normal distribution adequately fit the internal pressure strength data. Use this distribution and suppose that the sample mean of...

Why is it necessary or advantageous for the body to make zymogens?

Why is it necessary or advantageous for the body to make inactive hormone precursors?

Is the term KM used with allosteric enzymes? What about competitive and noncompetitive inhibition? Explain.

QUESTION 1 An investment has expected cash flows of -$200, $100, $220, $90 and $45 at the end of years 0 through 4, respectively. The required return is 8.5%. Using interpolation, estimate the...

Levi's Levees always evaluates projects using the payback method. What is the payback period for the following set of cash flows? (Round your answer to 2 decimal places, e.g., 32.16.) Cash Year Flow...

1.How does the Electoral College work? a. Congress selects presidential candidates; voters then elect the president. b. Each state determines how it will select its electors; electors then elect the...