Question: (a) Make a graph showing the retention time of each peak in Figure 24-25 in chromatograms B, F, and C as a function of position

(a) Make a graph showing the retention time of each peak in Figure 24-25 in chromatograms B, F, and C as a function of position along the line BC. Predict the retention times for solvent compositions midway between B and F and midway between F and C. Draw a stick diagram (representing each peak as a vertical line) of each of the two predicted chromatograms.
(b) What would be the solvent compositions midway between B and F and midway between F and C?

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a b B 40 methanol60 buffer C 32 tetrahydrofuran 68 buffer F 20 methanol16 tetrahydrofuran 63 buff... View full answer

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